no sprayer 01How to Clean a Closed Environment for Cells

Resisting the Misting

In a closed cell environment like a barrier isolator, there are few routes for disinfectants to leave and no way for contaminants to float in.

In an open laboratory, you can spray surfaces liberally with disinfectant and it all evaporates into the room air. Keeping surfaces wet with disinfectant for maximum contact time is the major concern with a closed cell environment.

It is a big change, moving from an open lab, which exists in a constant state of high microbial contamination risks, into the closed Xvivo system, a very low risk environment. A risk-based balance has to be struck between much lower risk of contamination and the higher risk of disinfectant fume build-up.

cytocentric visionaries jj p3Cytocentric Visionaries: Jan Jensen, Trailhead BioSystems 

Part Three: Full-time Oxygen Control and Aseptic Conditions are Critical for Automated Protocol Optimization

In Part Two, we discussed Trailhead’s unbiased system of cell signaling optimization for differentiation. Today, Dr. Jan Jensen. CSO of Trailhead BioSystems discusses full-time optimization of cell culture conditions with us. Read part three below to learn how automated lab systems can assist with GLP compliance.

cytocentric visionaries jj p2Cytocentric Visionaries: Jan Jensen, Trailhead BioSystems 

Part Two: Controlling Stray Cell Fates in Culture

In Part One, we discussed Trailhead’s unbiased system of cell signaling optimization for differentiation. Here we continue our conversation with Dr. Jan Jensen, CSO of Trailhead BioSystems in Cleveland, OH. Today we talk about cell fate and reprogramming. Read the full interview to learn about the impact of cell reprogramming in clinical applications.


cytocentric visionaries jj p1Cytocentric Visionaries: Jan Jensen, Trailhead BioSystems 

Part One: Unbiased Cell Science: Don’t Try to Out-Think Nature

Dr. Jan Jensen is the E. J. Brandon Endowed Professor in Diabetes Research at the Cleveland Clinic and also CSO of Trailhead BioSystems. Trailhead uses a unique combination of advanced technologies to define optimized combinations of growth factors that will efficiently guide cell differentiation from the embryonic state through specific cell lineage pathways.

Combining a Design of Experiments approach with robotics, mathematical modeling, high throughput gene analysis, and Cytocentric Isolators, Trailhead optimizes cell media 12 factors at a time to achieve step-by-step cell type conversion rates of well over 90%.

Here, Alicia talks with Dr. Jensen about how they had to let go of the past to progress. The part one interview below explores finding different signaling molecules with large scale computing and how it benefits scientific research.

bp xvivo headerProduction of Retinal Progenitor Cells under Full-Time Optimized cGMP Conditions in the Barrier Isolator

recent paper in Nature’s Scientific Reports from the Budd Tucker’s lab at University of Iowa reported producing xeno-free clinical-grade retinal progenitor cells from iPSC in a barrier isolator with cGMP reagents and conditions. [1] This is very exciting news for the iPSC field. Continue reading to learn about this research involving production of retinal progenitor cells in optimized cGMP conditions.