Dr. Hal Broxmeyer is a Distinguished Professor, Professor of Microbiology/Immunology and Co-Leader of the Program on Hematopoiesis, Malignant Hematology, and Immunology at Indiana University Simon Cancer Center. He has chalked up well over 1,000 scientific publications and his work has been seminal to the field of hematopoietic stem cell transplantation. His group’s 2015 paper in Cell and the subsequent review articleare landmark papers that demonstrate how room air oxygen exposure during cell isolation inflicts damage on freshly isolated stem cells.
Here, we talk with Dr. Broxmeyer about his work and learn how relevant oxygen conditions affect hematopoietic progenitor cells. This interview was conducted by email and edited for length.
The Lasker Awards were won this year by a trio of incredibly important figures in physiologic oxygen research; William Kaelin, Peter Ratcliffe, and Gregg Semenza.
What did they do?
An important new publication in Stem Cells and Development by Tiwari et al in Australia has brought a critically important in vitro oxygen diffusion issue to the forefront. In a murine model, they explore optimum tissue oxygenation levels for best in vitro expansion of HSC for transplant.
In a closed cell environment like a barrier isolator, there are few routes for disinfectants to leave and no way for contaminants to float in.
In an open laboratory, you can spray surfaces liberally with disinfectant and it all evaporates into the room air. Keeping surfaces wet with disinfectant for maximum contact time is the major concern with a closed cell environment.
It is a big change, moving from an open lab, which exists in a constant state of high microbial contamination risks, into the closed Xvivo system, a very low risk environment. A risk-based balance has to be struck between much lower risk of contamination and the higher risk of disinfectant fume build-up.
In Part Two, we discussed Trailhead’s unbiased system of cell signaling optimization for differentiation. Today, Dr. Jan Jensen. CSO of Trailhead BioSystems discusses full-time optimization of cell culture conditions with us. Read part three below to learn how automated lab systems can assist with GLP compliance.