cytocentric visionaries rv p1Cytocentric Visionaries: Romain Vuillefroy de Silly

Part 1: Is Physiologic Oxygen Better for CAR-T Cells In Vitro?

Top 5 Cytocentric Papers of 2016

In this post we highlight the top 5 publications for cell-centered research in 2016 that demonstrate the effectiveness of the Cytocentric approach for cell and tissue cultures. Here I specifically excluded papers in the field of Immunology because we will soon be publishing a separate post to highlight Cytocentric Immunology papers of 2016. Let’s count down the best of 2016:

cytocentric visionaries at p1Cytocentric Visionaries: Abhilasha Tiwari, PhD, Monash University

What do you look for when you want real answers? Real questions.

Dr. Abhilasha Tiwari is a SIEF-STEM+ Business Postdoctoral Researcher at The Ritchie Centre, Hudson Institute of Medical Research and Monash University. Her work with Graham Jenkin and Mark Kirkland focuses on optimal expansion of hematopoietic stem cells (HSC) from umbilical cord blood.

Here, we talk with Dr. Tiwari about her recent publication in Stem Cells and Development, “Impact of Oxygen Levels on Human Hematopoietic Stem and Progenitor Cell Expansion.” [1] This interview on how pericellular oxygen affects cord blood HSCs was edited for brevity.

Why Optimize MSC Cell Culture Conditions?

Feed a Fibroblast, Starve a Stem Cell

A new publication in Stem Cell Research & Therapy (open access) from Alan Wells’ group at University of Pittsburgh has some highly relevant findings for Mesenchymal stem/stromal cell (MSC) culture optimization. MSC are an incredibly valuable cell type for both industrial and research scale cell-based therapeutics. The Wells group reported that in their investigations of the relationships between MSC starvation, autophagy, and differentiation, they stumbled upon a strikingly high glucose consumption rate in comparison with other cell types.1

cytocentric visionaries sm p2Cytocentric Visionaries: Shannon Mumenthaler, University of Southern California

Part Two: Oxygen, Cell Morphology, and Time

In Part One of this two-part interview, we talked with Dr. Shannon Mumenthaler about her latest studypublished in Nature’s Open Access Journal, Scientific Reports [1] and her unique combination of high throughput image analysis, heterogeneous cell culture, and full-time control of conditions. Today we talk about adding new dimensions into highly dimensional cell parameter space, including cell shape and time. Read the full interview transcripts below to learn more about how cell morphology can be used to better understand cancer biology.