• Complete Oxygen Profiling
• Multiple Setpoints & Chambers
• Full Range Oxygen Control 0.1-99.9%
• Simulate Any Dynamic Physiological Condition

Changes in oxygen concentration affect the physiology of your cell cultures. But it can only be studied if it can be recreated. To recreate these physiologic conditions, such as heart attacks, strokes, and epilepsy oxygen profiles need to be created. Profiles have multiple setpoints which change at precise times with any rate of change from one setpoint to the next.

BioSpherix, Ltd. offers oxygen profiling with our sophisticated OxyCycler O2 and/or CO2 controller. You can easily create any profile you need with full range oxygen control (0.1% to 99.9%) and/or CO2 control (O.1% to 20.0%). The OxyCycler independently controls in multiple chambers and can hold any setpoint for any length of time, manipulate any rate of change between setpoints, and cycle any profile any number of times in any chamber. With the OxyCycler, complex oxygen profiling is made easy.

HOW IT WORKS

The OxyCycler sits outside your incubator and is attached to the chambers within the incubator by umbilicals with actuator pods on the ends. The pods contain the oxygen sensor, a gas nozzle and mounting hardware.

Our C-Chambers have specifically designed adapter plates for the pods to mount to. The OxyCycler remotely senses the oxygen concentration in the chamber and infuses either oxygen to increase concentration or nitrogen to lower concentration to reach your setpoint.

The OxyCycler comes with a computer system for an easy interface, real-time trend charting, data logging and remote operation.

Since the OxyCycler works in semi-sealed chambers, the profiles are normobaric. Nitrogen and oxygen gas infusions displace the chamber gas and pressure remains equilibrated with outside pressure.

Any gas supply can be utilized by the OxyCycler but some supplies may be more efficient. Compressed is best with low consumption, generators are best with high consumption and liquid is best in the middle.

PROFILES

The OxyCycler allows you to create any change in oxygen and/or carbon dioxide concentration. You can independently control and profile up to four chambers simultaneously. Which means you have one control profile and up to three experimental profiles.

Up to 17 different profiles can be stored and each can be run in any of the four chambers at any time. Chambers can have the same profile simultaneously or staggered to begin at different times.

Each profile can have 1 to 20 setpoints and can be from 0.1% to 99.9% oxygen. The rate between setpoints can be 0 - 999 minutes with resolution to seconds. Profiles can be cycled continuously or 1 to 99 times. Examples of profiles are shown at right.