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XVIVO SYSTEM

Cytocentric-by-Design ushers in new era for cell scientists at the leading edge!

 


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Smallest, simplest Xvivo System configuration consisting of one closed hood, integrated with one closed incubator. Just one of hundreds of possible configurations.

 

 

NEW AND BETTER EQUIPMENT
Conventional incubators and hoods haven't changed in decades. They are limited in ability. They were designed for people, not cells. Now a radical new alternative type of equipment is available for manipulating and growing cells. It is unlike any other equipment. The Xvivo System is the first system designed to go beyond the ordinary needs of people and specifically address the advanced needs of cells. Better for your cells. Better for you.

FROM THE PERSPECTIVE OF CELLS
When you look through the eyes of your cells, handicaps inherent in conventional People-Centric equipment are obvious. Protection from contamination is poor. Critical parameters are not accounted for, or poorly controlled at best. Eliminating these handicaps and better meeting the needs of cells requires something different. For people, the Xvivo System may be a radical new concept. For cells, it's not. For cells, it is about time.

CELL INCUBATION AND PROCESSING RE-INVENTED
In contrast to conventional cell culture and processing in open incubators and open hoods, the Xvivo System consists of modular sets of closed incubators and closed hoods, all integrated together as co-chambers and sub-chambers. Modularity allows an infinite variety of different configurations. All are completely closed, with aseptic conditions throughout, and advanced controls wherever needed. Microscopes, centrifuges, sorters, and all other common cell tools can be integrated as well. For the first time, cell needs can be met by one efficient system.

 

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CLOSED HOODS with integrated incubation chambers are the key design feature. Incubators open only into aseptic space. Contamination risk is dramatically lower. Hood is also controlled with all cell parameters, same as incubator. When cells are removed from incubator, they experience no disruption in temperature or CO2 or pH or O2 or etc. Technicians work comfortably in soft flexible clear plastic gloved windows, isolated from cells.

 

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CLOSED "LAB BENCH" End view looking down through several interconnected Xvivo processing chambers. Processing and manipulating cells, whether manually or automatically, is done same way as on open lab bench or inside conventional open BSL hood. Difference is superior conditions for cells in Xvivo System.

 

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MODULAR & SCALABLE The above Xvivo System displays the unique ability to expand and change focus as your research progresses. Easily add a microscope chamber, buffer chamber and safety hood as needed.

 

HIGH PERFORMANCE INCUBATORS
Incubation chambers are integrated into the hoods where you need them, and open only into the hood. This eliminates 2 major handicaps in conventional open incubators and open hoods. First, incubators can't get contaminated by people or by the room. They are isolated from both. They open only into the aseptic hood. Second, cells never see any disruptions in critical cell parameters, because all cell parameters controlled in incubators are also identically controlled in hoods. Any number of independent incubation chambers can be fitted into a system. Each can be sized for and dedicated to each different culture to accommodate any number of different culture protocols simultaneously. Basic cell parameters like O2 are fully accounted for, and exotic new cell parameters not available elsewhere provide better simulation of physiologic conditions. Every parameter can have dynamic control to keep up with the changing dynamics of any cell population.

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EXPANSION PROTOCOL - Primary cultures and other low cell population density do best under low oxygen gas phase when they start, but when they grow, they are bound to need more. You can design any ramp up in oxygen, and consistently repeat it. With a click of the mouse.

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ACUTE/INTERMITTENT HYPOXIA - Hypoxic stress can model components of many severe diseases such as heart attacks, strokes, asthma, or epilepsy. Frequency, duration and degree of drops are all adjustable.

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HYPOXIC PRECONDITIONING - Cells destined for implantation will experience hypoxia and may be better prepared if they are conditioned to it before implantation. Ischemia may also be protected by conditioning. The OxcyCycler C42 can easily run any preconditioning profile.

 

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HYPOTHETICAL PROTOCOL - To illustrate the unprecedented power of dynamic multi-variable optimization. Imagine you have a transgenic cell population to be implanted. You use the last few hours before implantation to prepare it. You know the cells are going to better survive the time between the dish and the body if their metabolism slows down, so you program the temperature to gradually cool the cells just before they leave the incubator. You know your cells are going to be hypoxic for their first few days in the body, so you program the O2 for a series of acute hypoxic episodes in the last hours before implantation to precondition them, then at the last moment make the O2 rise to saturate their media just before they leave the incubator. You also know they will handle the stress a lot better if you can upregulate their stress response, so you program a short burst of CO just before they leave the incubator. As usual, you program the CO2 concentration to stay constant while the other variables are manipulated up and down.

 

HIGH PERFORMANCE INTEGRATED HOODS
Hoods are closed instead of open. Isolation of cells away from people and the room cuts risk of contamination during handling dramatically. Multiple levels of aseptic conditioning provide cleaner cell environment than any conventional BSL Class 100 hood. All manual and automated processing is protected from contamination. All incubators are protected because they open only into these isolated protected aseptic hoods. All cells are protected. All the time. Hoods can have some or all the same cell parameters as the incubators. Now it is easy to handle and process cells with no disruption in optimum conditions.

UNLIMITED FLEXIBILITY

Xvivo System modularity means you can invest with confidence. Start small and expand as necessary. Reconfigure if your needs change. No dead ends.

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Xvivo System can grow with you. Modularity allows you to invest in just what you need now, and then add to it later when your needs change or expand.

  • STEP ONE: Starter system with one closed hood for culture processing and one incubation chamber on end.
  • STEP TWO: Same starter system: moved incubator to back wall, added a microscope chamber (open and contiguous with culture processing chamber), and another processing chamber for second technician and 3rd party equipment like cell separator, colony picker, plate reader, etc.
  • STEP THREE: Added 12 additional incubation chambers (8 in one hood, 4 in the other) for a new total of 13, each independently controlled, and added a couple of small laminar flow clean hoods one at each end. Modularity means you will never be limited.
 

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"It is an outstanding system."

Mark Mercola, Ph.D.
Associate Director,
Stem Cell
Research Center
Burnham Institute for Medical Research

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