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CELL CULTURE INCUBATOR... THE WORKHORSE OF THE CELL BIOLOGY LAB... and has been for decades.

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People-centric design...
Cytocentric limitations

People love their cell culture incubators.

 

Laboratory appliance manufacturers have been improving cell culture incubators for probably almost 100 years.

INDUSTRIALLY HARDENED:  Incubators are well built. They are reliable. They are simple to operate.

ECONOMICAL: They are economical. Manufacturers achieve huge economies of scale in material, labor, overhead by mass producing similar appliances such as freezers, refrigerators, hoods, ovens, etc.

EASY OPERATION: Incubators are easy to operate. Simple to use. As convenient as your refrigerator at home.

LATEST INNOVATION: Self decontamination. Coved corners and removable autoclaveable shelves and racks. Now dry heat cycle approaching self autoclave and internal HEPA cleaning.  99% of cells outside the body spend 99% of their time in incubators.

 

However, sometimes cells need more!

 

Sometimes Cells Need Better Physiologic Simulation

CO2 incubators do not control O2. The O2 concentration inside is fixed near  the ambient air level (21%). Diluted slightly with the CO2 and humidity, the O2 level (18-20%) is maybe 10 x higher than physiologic levels. Incubating cells at physiologic O2 tensions results in more normal phenotype and more relevant data.

CYTOCENTRIC SOLUTION #1 - Change the ambient O2 level to a lower physiologic level. Simply insert and operate your incubator inside the I-Glove with physiologic O2 control. I-Glove is the first and only glove box designed for housing incubators. Retains entire volume of incubator. Additional advantage of hands-on uninterruptibility, low gas consumption, and contamination protection from closed system. (CO2 incubator + I-Glove )

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CYTOCENTRIC SOLUTION #2 - Integrate your CO2 incubator into an Xvivo System with physiologic O2 control. Also retains entire volume of incubator. Additional advantage of hands-on uninterruptibility, low gas consumption, contamination protection from closed system, and full integration of any other incubation and processing procedures and any 3rd party equipment. (CO2 incubator inside Xvivo System module)

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CYTOCENTRIC SOLUTION #3 - Upgrade with SubChamber Culture System add on accessory  that controls physiologic O2 inside, isolated from the rest of the incubator. Additional advantage of space efficiency, gas efficiency or low gas consumption, and hands-off uninterruptibility. Opening incubator door will not disturb physiologic O2 or hypoxic O2. (CO2 incubator + ProOx C21 with C-Chamber)

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CYTOCENTRIC SOLUTION #4 - Directly add O2 control to the CO2 incubator. Upgrade CO2 incubator with ProOx modular O2 controller. Transforms it into O2/CO2 incubator, as good as any with O2 control already built in, and better than most. Better because of wider range of O2 control including deep hypoxia down to 0.1%, which most built in O2/CO2 incubators can't do. It is cost efficient because you don't have to buy a new incubator. Space efficient  because you don't have to fit an extra incubator in the lab.(CO2 incubator + ProOx 110)

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Sometimes Cells Need Full Time Optimization

...or steady, continuous, uninterrupted conditions. One of the things people love about standard incubators is the simple convenience of opening and closing them just like the refrigerator at home. However, each time the door is opened all the optimized cell parameters are disrupted. Temperature, pH, CO2, and O2 are all disturbed, and cultures experience suboptimal conditions as room air rushes in. Multi-user incubators in a busy lab can be opened dozens of times per day. If a technician has to spend some time looking for certain cultures, or gets distracted while the incubator door is opened, sometimes these disruptions are lengthy. New knowledge of a large number of O2 regulated genes and their extreme sensitivity to even small fluctuations makes this especially worrisome for phenotype integrity. Steady, continuous, uninterrupted conditions reduce variability and results in more consistent phenotype. In hypoxia simulations it is usually the difference between success or failure.

CYTOCENTRIC SOLUTION #1 -  For steady continuous uninterrupted O2 and CO2 put your O2/CO2 incubator inside an I-Glove with identical O2 and CO2 levels. This way every time you open the incubator door, there is no change in either O2 or CO2 levels. Retains entire volume of incubator. One additional advantage is hands-on uninterruptibility so you can process and handle your cultures at same O2 and CO2. Second additional advantage is greatly reduced gas consumption because the incubator no longer has to recover after a door opening. Third additional advantage is reduced risk of contamination by closing incubator inside aseptic environment. (O2/CO2 incubator + I-Glove)

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CYOCENTRIC SOLUTION #2 - Integrate your O2/CO2 incubator into an Xvivo System. Retains volume of incubator. No change in O2/CO2 when open incubator door. Hands-on uninterruptibility. Low gas consumption. Reduced contamination risk. Unlimited integration options with any other incubation or processing procedures or equipment.

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CYTOCENTRIC SOLUTION #3 - Add SubChamber Culture System for hands-off uninterruptibility.

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CYTOCENTRIC SOLUTION #4 - Add SubChamber Culture System and C-Shuttle Glove Box for hands-on uninterruptibility.

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Sometimes Cells Need Different Protocols

Conventional cell culture incubators are monolithic chambers with a single set of conditions. Often many different cultures with possibly different requirements have to be put in the same incubator and cultured under identical conditions. Cells with different requirements suffer. Providing optimum conditions for different protocols improves everything - growth, health, vitality, phenotype integrity, data relevance.

CYTOCENTRIC SOLUTION #1 - Add SubChamber Culture System accessory to your incubator and provide exactly the conditions each different culture requires. You can have as many different protocols as the number of SubChambers you can fit inside your incubator.

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Incubator + SubChamber for 2 protocols

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2 subchambers for 3 protocols

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3 subchambers for 4 protocols

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4 subchambers for 5 protocols

 

Sometimes Cells Need Dynamics

Conventional cell culture incubators provide only static conditions. Yet sometimes cells need dynamic conditions because they change over time. The classic example is the O2 deprivation that occurs in expanding cell population cultures, and increased acidity from lactic acid production. You can enhance cell expansions by dynamically raising the O2 over the course of the culture to prevent O2 deprivation, and dynamically lowering the CO2 to counter the acidity. If your cells are doubling every day and you get one additional day, you double your expansion. More complex dynamics can provide better simulations of pathophysiologic phenomena and create special phenotypes. 

CYTOCENTRIC SOLUTION #1 - Directly add dynamic control to any existing incubator. Modular retrofitting O2 and/or CO2 controllers such as the OxyCycler can fit any make or model, thermal incubators, CO2 incubators, O2/CO2 incubators. Retains volume of incubator, no matter how large.

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   (incubator + OxyCycler C42 = dynamic control)

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  (side-by-side dual incubator + OxyCycler C42 = dual dynamic control)

 

CYTOCENTRIC SOLUTION #2 - Add SubChamber Culture System with dynamic control. Space efficient and gas efficient. Appropriate for smaller cultures.

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   (Incubator + OxyCycler C42 + 2  C-Chambers )

 

Sometimes Cells Need Better Protection

Conventional cell culture incubators open into the room. Technicians stand face to face with plates flasks etc. when incubator door is open. They possibly cough, sneeze, talk when accessing cultures with no barrier to germs landing in the warm humid environment full of nutrient media, fertile for microbial growth. In fact, contamination risk of open incubators is so great the regulatory agencies will not allow them to be used outside of cleanrooms with complete aseptic garb on all techs for any GMP application (cell therapy). However you can now better protect cells incubating in conventional cell culture incubators:

CYTOCENTRIC SOLUTION #1 - Integrate incubator into Xvivo System. Keeps incubator inside aseptic system. Absolute highest level of protection possible, better than even cleanrooms. Retains volume of incubator. (incubator + Xvivo module)

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CYTOCENTRIC SOLUTION #2 - Insert incubator inside I-Glove. Closed system with aseptic conditioning decreases contamination risk. (Incubator + I-Glove )

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CYTOCENTRIC SOLUTION #3 - Add SubChamber Culture System to incubator and shuttle closed chamber back and forth to C-Shuttle Glove Box without exposing to room air or technicians. (Incubator +SubChamber + CShuttle )

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SOMETIMES CELLS NEED MORE... than people!

Have something in mind...ready to upgrade >

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Xvivo Systemwith integrated microscope chamber
and four third party cell culture incubators built into the back.

 

 

"The Information we gain with the Xvivo System is important to our mission of developing therapies to improve patients' lives."

The Wake Forest Institute for Regenerative Medicine Winston-Salem, NC
www.wfirm.org

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