The unique C-Shuttle Glove Box is a component of the SubChamber System. It provides a workspace for cell culture processing under the same O₂/CO₂ conditions that the cells live, while in the incubator.

Another component, the C-Chambers, provide a gas tight transport of cultures from the incubator to the workspace and back to the incubator

The pass through chamber, or antechamber, of the glove box is designed to fit C-Chambers. You can leave C-Chambers in the antechamber, and remove cells into uninterrupted O₂/CO₂ atmosphere, manipulate them without disturbance, and finally return them into the incubator with the same O₂/CO₂.

 

One C-Shuttle Glove Box can handle dozens of distributed C-Chambers around the building. You just have to take turns.

The C-Shuttle is made of white clean chemical resistant polypropylene. It is simple, practical and effective.

An undisturbed cell is likely a happy cell

Typical Configuration

C-Shuttle Glovebox with Oxygen Control

Oxygen Glovebox for cell culture

 

C-Shuttle Glovebox with Oxygen and Carbon Dioxide Control

  CO2 Glovebox for cell culture

 

C-Shuttle Operation

STEP 1 - Balance C-Chamber in one hand while you open pass through door with the other. It’s safer to set C-Chamber down while you open pass through.

 

STEP 2 - Slide front of C-Chamber into antechamber (pass through chamber). Be careful to keep C-Chamber level while you place it inside.

 

STEP 3 - Set C-Chamber all the way inside, so front door nudges up against the workspace door. Then close pass through door.

 

Uninterruptibility

When working in a C-Shuttle your cells are never exposed to sub-optimal conditions. Whether you’re using Oxygen/CO₂/Nitric Oxide your workspace and transport is always controlled to a precise and optimal level. Top scientists have been telling us for decades that the sudden shift of oxygen from open incubator doors and manipulating cells in normal room air is causing havoc on HIF and other transcription factors.

 

5% of the Genome is regulated by oxygen.

Clinical evidence is mounting regarding oxygen's role in gene expression. Difference in culture and processing conditions lead to inconsistent results. Differences in culture and processing conditions leads to inconsistent results.

Contamination

In open labs with open hoods, cells and researchers share the critical workspace most of the time. The majority of contamination in labs is caused by humans and their bioburden. With a closed environment  provided with the C-Shuttle and C-Chambers the main source of contamination is removed.

Humans and Their Bioburden A person standing motionless in a Cleanroom can generate 100,000 particles 0.3 micron and larger, while a person moving at 2-5 mph can generate 10,000,000 particles 0.3 micron and larger.  Theobold, D. USP797: “Personal training” CLEANROOMS p.14 March )%

C-Shuttles protect the researchers

Cells are often purposely grown and processed to generate or test viruses and vectors. More worrisome is when cells are unknowingly infected with viruses or other transmissible agents. C-Chambers will limit exposure to harmful viruses, vectors and prions where conventional open labs allow the technicians to breathe potentially dangerous non-cellular biological entities.

 

Softside gloves are most comfortable and flexible. Iris ports are also an option. Two sets of gloves allow two different people to access the workspace at the same time.

C-Shuttle Performance

C-Shuttle antechamber receives chambers C-174, C-274, and C-374. Cultures can easily be moved from their host incubator, without disturbance of O₂ and/or CO₂.

 

Cultures can be handled inside the glove box workspace at the same O₂ and/or CO₂ by simply opening the C-Chamber from inside.

 

Lighting options available.