Cytocentric Blog

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Cytocentric Visionaries: Lauren Zenewicz, University of Oklahoma Health Sciences Center

Don’t Miss Your Cells’ Phenotype by Using Inappropriate Room Air Oxygen

Dr. Lauren Zenewicz is an Assistant Professor in the Department of Microbiology and Immunology at the University of Oklahoma Health Sciences Center. Her lab studies Interleukin-22 and its role in the inflammation and disease. She published a recent article in Frontiers in Immunology on in vitro oxygen levels during immunological studies1. In this article, she proposes that oxygen be considered a microenvironmental trigger for immune cell signaling.

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Cytocentric Visionaries: Roger Rönn

The Cell Environment for HSC from iPS: Why Every Hurdle Must be Overcome to Reach our Destination

This is an update on a previous interview that Dr. Alicia Henn, Chief Scientific Officer of BioSpherix, conducted with Roger Rönn, who recently finished his PhD and is now working in the laboratory of Elaine Dzierzak at the University of Edinburgh. Here we discuss his most recent paper in Stem Cells 1. This interview was edited for length.

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Cytocentric Visionaries: Romain Vuillefroy de Silly

Part Two: What’s in a Word? If It Describes the Oxygen Level, Quite a Bit.

Dr. Romain Vuillefroy de Silly is a post-doc at the Ludwig Center for Cancer Research of the UNIL in Lausanne, Switzerland. He has published original research papers and several extensive reviews on the role of oxygen in T cell function. This is Part Two of a Two-part interview that Dr. Alicia Henn, Chief Scientific Officer of BioSpherix, conducted with him about his most recent review in Oncoimmunology. [1] This interview was edited for length.

Part One of our interview can be found here: Is Physiologic Oxygen be Better for CAR-T Cells In Vitro?

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Cytocentric Visionaries: Romain Vuillefroy de Silly

Part 1: Is Physiologic Oxygen Better for CAR-T Cells In Vitro?

Dr. Romain Vuillefroy de Silly is a post-doc at the University Ludwig center for Cancer Research of the UNIL in Lausanne, Switzerland. He has published original research and several compelling reviews on the role of oxygen in T cell function. Here is a written interview Dr. Alicia Henn, Chief Scientific Officer of BioSpherix, conducted with him about his most recent review in Oncoimmunology. [1] This interview was edited for length.

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Top 5 Cytocentric Papers of 2016

In this post we highlight the top publications of 2016 that illustrate the Cytocentric approach to cell and tissue culture. Here I specifically excluded papers in the field of Immunology because we will soon be publishing a separate post to highlight Cytocentric Immunology papers of 2016. Let’s count down the best of 2016:

 

#5

Low Oxygen Modulates Multiple Signaling Pathways Increasing Self-Renewal while Decreasing Differentiation, Senescence and Apoptosis in Stromal MIAMI Cells.This paper in Stem Cells and Development [1] (paywalled), Rios et al pull together multiple pathways affected by culture oxygen levels in a subtype of immature MSC called marrow-isolated adult multilineage inducible (MIAMI) cells. Their comparison of 21% and 3% oxygen for MIAMI cell culture helped elucidate how specific signal transduction mechanisms produce the observed profound effects of oxygen on cell cycle and differentiation.

It is a small point, but one of the best things about this paper is that the researchers grew the cells for 7 days at physiologically relevant oxygen before their measurements. It has been a frequent disappointment to read a paper that promised results in physiologically relevant culture to find that the reseachers only had their cells in the appropriate conditions for 24 hours (or less!) before their endpoints. The use of these short incubation times is misleading in that it gives us a snapshot of cells in transition to their new environment rather than a steady-state. The authors of this paper understand oxygen biology enough to avoid this mistake and have contributed a key piece to our picture of how culture oxygen levels affect stem cells.

 

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