ProOx C21 Pod:

INCREASE CIRCULATION & DECREASE INCUBATOR CONTAMINATION
The pod component of the ProOx C21 is uniquely designed to isolate the critical cell environment which can get contaminated from the sensing mechanisms for monitoring the gases in the chamber. This isolation is accomplished by putting the sensors inside the pod and pumping a sample from the chamber to the pod then through the sensors back into the chamber.

Pod design isolates potential sources of contamination

This side stream sampling starts with the sterile filter and ends with a sterile filter inside the chamber so that if there was contamination it doesn’t get past the contaminated sensors. Likewise if there was a contamination of the sensors it wouldn’t contaminate the chamber.

C21 infusion pod with microbial filtration and homogenization fan.

Front Panel Interface

Typical Configuration

From outside the host chamber, the ProOx C21 works by remotely sensing oxygen and carbon dioxide inside the chamber and infusing gas to control both. O₂ is infused to raise the oxygen and N₂ is infused to lower it. CO₂ is infused to raise carbon dioxide and N₂ is infused to displace it.  Once the initial gas reaches its setpoint, the second quickly follows. The accessory pod that attaches to the C-Chamber SubChamber contains the gas sensors. These sensors are isolated behind microbial barriers and also isolated from the controlled environment of the culture chamber. The pod also provides the infused, sterile, filtered gas. A flexible conduit (umbilical) connects the controller to the pod.

The ProOx C21 consists of 2 basic parts connected by a closeable umbilical. The controller can be positioned where accessible by the user and the pod interfaces directly with the controlled unit or BioSpherix C-Chamber. The face of the accessory pod has a gas homogenizing fan and also provides the outlet of the gas infusion nozzle and side stream sampling ports for the protected sensors.

 

All operations are conveniently located at the front of the panel and all connections are located at the back and out of the way allowing for less interference and increasing usability for cell culture research and development.

O₂ and CO₂ concentration sensor readings are displayed in bright green numbers to be continuously and easily monitored from across the lab. Manual switch for gas provides a convenient shut off thus saving gas when chamber door is opened. An alarm buzzer, also manual, will remind you if you have forgotten to turn the gas back on.

Use Any Gas Supply:

ProOx C21 conveniently utilizes gas from any source. Best supply depends on consumption. Compressed gas is best in low consumption applications. Generator is best in high consumption applications. Liquid is best in between.

SAVES GAS AND MONEY:

Chamber gas consumption varies widely, but in every case the ProOx C21 always and absolutely minimizes gas consumption. It’s maximally efficient! Gas costs are reduced to an absolute minimum.

1. Sensors are isolated away from the cell culture space with microbial filters using disposable, user replaceable, microbial barrier filters.
2. The gas is homogenized by a small, user-sterilizable fan.
3. A cleaner look is enabled using a single complete umbilical rather than multiple individual components.
4. Easier to service...remove the cover of pod with a screwdriver, pop out sensor.
5. Easier service of the pod without breaching the integrity of the controlled chamber.

 

 

At time (1) user opened and closed door briefly for access. Room air rushed in and took both O₂ and CO₂ away from setpoint. However, automatic feedback immediately responded and returned both gasses back to setpoint. At time (2) user unlatched door but before he could open it became distracted and forgot to re-latch, thus increased chamber leakage.  Again however, automatic feedback control quickly adjusted and re-established setpoints. Obviously gas consumption increased to accommodate the increased load but the integrity of the experiment was maintained.